1 Japanese style diet นางสาววรรณชนก บุญชู Tokyo University of Agriculture and TechnologyDepartment of Applied Molecular Biology and BiochemistryMajor field of Physiological and Biological Chemistry Laboratory of Nutritional Biochemistry
2 50.06 53.96 79.00 85.81 World life expectancy at birth 2006 1947 2007 male female1Japan82.679.086.12Hong Kong (PRC)82.279.485.13Iceland81.880.283.34Switzerland81.784.25Australia81.278.983.66Spain80.977.77Sweden78.783.08Israel80.778.582.89Macau (PRC)10France (metropolitan)77.184.111Canada78.382.912Italy80.577.583.513New Zealand78.214Norway77.882.515Singapore80.078.081.916Austria79.876.917Netherlands18Martinique79.576.582.319Greece20Belgium1947male female2007
7 Glutamin:fructose-6-phosphate amidotransferase (GFAT) O-linked N-AcetylglucosamineO-GlcNAc is an abundant post-translational modification ofserine and threonine. residues of nucleocytoplasmic proteinsGlucoseGlycogen synthesisG-6-PGlycolysisF-6-PGlutamin:fructose-6-phosphate amidotransferase (GFAT)GlcN-6-P●Expression of O-GlcNAc modified-protein●Expression of O-GlcNAc related-enzyme●Purification of O-GlcNAc modified-protein-Complex glycosylation -Nucleotide synthesisUDP-GlcNAcO-GlcNAc transferase (OGT)①Western Blotting②RT-PCR③ImmunoprecipitationO-GlcNAcaseprotein O-GlcNAcylated protein
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