งานนำเสนอกำลังจะดาวน์โหลด โปรดรอ

งานนำเสนอกำลังจะดาวน์โหลด โปรดรอ

Clinical use of hormone status determination in canine and feline reproduction Suppawiwat Ponglowhapan, DVM (Hons), M.S., M.Sc, PhD Department of Obstetrics.

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งานนำเสนอเรื่อง: "Clinical use of hormone status determination in canine and feline reproduction Suppawiwat Ponglowhapan, DVM (Hons), M.S., M.Sc, PhD Department of Obstetrics."— ใบสำเนางานนำเสนอ:

1 Clinical use of hormone status determination in canine and feline reproduction Suppawiwat Ponglowhapan, DVM (Hons), M.S., M.Sc, PhD Department of Obstetrics Gynaecology and Reproduction Faculty of Veterinary Science, Chulalongkorn University Suppawiwat Ponglowhapan, DVM (Hons), M.S., M.Sc, PhD Department of Obstetrics Gynaecology and Reproduction Faculty of Veterinary Science, Chulalongkorn University

2 Introduction Hormone Hormone  Hormaein ( ภาษากรีก ) = Impetus (a moving force, stimulus) แปลว่า กระตุ้น (excite), ก่อกวน (stir up) หรือทำ ให้เกิดการเปลี่ยนแปลง (to set in motion) Endocrine Endocrine The endocrine system is a group of glands that work together and secrete many types of different hormones that regulate the body.

3 Introduction Major endocrine glands. (Male on the left, female on the right.) 1. Pineal gland 2. Pituitary gland 3. Thyroid gland 4. Thymus 5. Adrenal gland 6. Pancreas 7. Ovary 8. TestesMalefemalePineal glandPituitary glandThyroid gland ThymusAdrenal glandPancreasOvaryTestes

4 Introduction การควบคุมการทำงาน ▫ Autocrine ▫ Paracrine ▫ Endocrine

5 Introduction Hormone feedback regulatory system ▫ Negative feedback ▫ Positive feedback Physiological mechanisms of endocrine Pathologic mechanisms of endocrine ▫ Hormone excess (tumor, exogenous route) ▫ Hormone deficiency (autoimmune, surgery, inflammation) ▫ Hormone resistant (genetic factor)

6 Introduction สามารถแบ่งฮอร์โมนออกเป็น 5 ชนิด 1. Amino acid derivatives; dopamine, catecholamines, thyroid hormone 2. Small neuropeptides; GnRH, TRH, somatostatin, vasopression 3. Large proteins; LH, FSH, insulin, parathyroid hormone 4. Steroid hormones; oestrogen, progesterone, testosterone, cortisol 5. Vitamin derivatives; retinoids

7 Introduction หน้าที่หลักของฮอร์โมน 1.Growth and differentiation 2.Maintenance of homeostasis 3.Reproduction

8 Introduction สามารถแบ่งฮอร์โมนออกเป็น 5 ชนิด 1. Amino acid derivatives; dopamine, catecholamines, thyroid hormone 2. Small neuropeptides; GnRH, TRH, somatostatin, vasopression 3. Large proteins; LH, FSH, thyroid hormone, insulin 4. Steroid hormones; oestrogen, progesterone, testosterone,cortisol 5. Vitamin derivatives; retinoids

9 Reproduction Progesterone Oestrogen Testosterone Gonadotrophins: LH & FSH Thyroid hormone

10 Progesterone Definitions 1. A female hormone and the principal progestational hormone that is made mainly by the corpus luteum in the ovary and by the placenta. Progesterone prepares the lining (endometrium) of the uterus to receive and sustain the fertilized egg and so permits pregnancy. 2. The principal progestational hormone liberated by the corpus luteum, adrenal cortex, and placenta, whose function is to prepare the uterus for the reception and development of the fertilized oocyte by inducing transformation of the endometrium from the proliferative to the secretory stage.

11 A sex steroid hormone CL, adrenal gland, placenta (cat) Pregnancy, mammary gland development Progesterone

12 ประโยชน์ของการตรวจโปรเจสเตอโรนในสุนัข 1. เพื่อกำหนดวันผสม (optimal breeding time)  เพิ่มโอกาสผสมติด  เพิ่มจำนวนลูกต่อครอก 2. เพื่อตรวจความผิดปกติของการตั้งท้อง 3. เพื่อทำนายวันคลอด

13 Determination of optimal breeding time in the dog

14 Luteinization of the follicle in the dog

15 Guidelines for interpretation of serum progesterone concentration in the estrous bitch measured by RIA Serum Progesterone (ng/mL) Estimated day of ovulation Best breeding daysEstimated parturition from ovulation days days+ 4 days (3-6)+ 63 days (62-64) days+ 3 days (2-5)+ 63 days (62-64) days+ 2 days (1-4)+ 63 days (62-64) For natural mating or AI using fresh semen, inseminate the day after ovulation For AI using chilled or frozen semen, inseminate 2-4 days after ovulation

16 Predicted time of ovulation based on serum progesterone (n = 50; > 20 breeds) Key points 1.Traditional or classical dates for canine breeding: No one-for-all rule 2.Importance of determining serum progesterone level for canine breeding

17 Determination of optimal breeding time in the dog

18 LH and ovulation? Why LH check is less likely to be recommended in practice? ▫ Specificity of LH test ▫ Lab Availability :Thailand? Determination of optimal breeding time in the dog

19 Is there any in-house LH test kit? Determination of optimal breeding time in the dog YES

20 Some considerations on the clinical use of LH test kit ▫ Advantages ▫ Price ($230 per box/ 6 tests) ▫ The number of cases ▫ Frequency of the use/ cycle Determination of optimal breeding time in the dog

21 Unlike other mammals, dogs ovulate when the oestrogen level is declining. onlygeneral idea Oestrogen levels can only give us a general idea of when a dog will come into cycle. Determination of optimal breeding time in the dog

22 Vaginal cytology in the cat

23 Oestrogen and vaginal cytology? basal/ parabasal cell intermediate cell superficial cell Determination of optimal breeding time in the dog

24 The use of vaginal cytology for canine breeding management ▫ To ensure a dog is in cycle ▫ When to start vaginal swab? ▫ How often? ▫ When to start collecting blood sample for progesterone assay? Determination of optimal breeding time in the dog

25 Recommended workup for determination of optimal breeding time in the bitch History taking History taking (previous oestrus, first day of vaginal bleeding: Day0) Physical exam Physical exam (General and reproductive exam; vaginal discharge, vulvar swelling) Estrous behaviors Estrous behaviors Vaginal smear Vaginal smear to confirm the bitch is in estrous stage Blood collection Blood collection for progesterone measurement

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27 Guidelines for interpretation of serum progesterone concentration in the estrous bitch Serum Progesterone (ng/mL) Estimated day of ovulation Best breeding daysEstimated parturition from ovulation days days+ 4 days (3-6)+ 63 days (62-64) days+ 3 days (2-5)+ 63 days (62-64) days+ 2 days (1-4)+ 63 days (62-64) Key Notes 1.Stress and progesterone hypersecretion 2.Combination of behaviour, vaginal cytology and progesterone 3.Delivery of live puppies can not be guaranteed.

28 Progesterone ประโยชน์ของการตรวจโปรเจสเตอโรนในสุนัข 1. เพื่อกำหนดวันผสม (optimal breeding time)  เพิ่มโอกาสผสมติด  เพิ่มจำนวนลูกต่อครอก 2. เพื่อตรวจความผิดปกติของการตั้งท้อง 3. เพื่อทำนายวันคลอด

29 Pregnancy monitoring

30 Hypoluteoidism = luteal insufficiency Progesterone levels > ng/mL are necessary to support pregnancy. Incidence? Cause? ▫ Insufficient relaxin low prolactin low progesterone ▫ Endometrial infection/inflammation PGF2 alpha luteolysis Hypoluteoidism in the bitch

31 Treatment ▫ Medroxyprogesterone acetate (MPA) 0.1 mg/kg PO sid ▫ Altrenogest mg/kg PO sid ▫ Progesterone in oil 1-2 mg/kg IM every other dayNOTE Discontinued 2-3 days prior to the expected due date

32 Progesterone ประโยชน์ของการตรวจโปรเจสเตอโรนในสุนัข 1. เพื่อกำหนดวันผสม (optimal breeding time)  เพิ่มโอกาสผสมติด  เพิ่มจำนวนลูกต่อครอก 2. เพื่อตรวจความผิดปกติของการตั้งท้อง 3. เพื่อทำนายวันคลอด

33 Prediction of parturition date

34 กำไร 3 Oct Oct 2009 Golden Retriever, 2 yrs 54 days of pregnancy Severe bloody vaginal discharge General health status is good No signs of parturition X-ray: 3 fetal skeletons (not yet full-term) U/S: one fetus with slow heat beat (< 180 bpm) ▫ Predicted parturition date by U/S (biparietal diameters) = -7 days

35 6 Oct Oct 2009 Abortion: one fetus Progesterone level 1.9 ng/mL 7 Oct 2009 at night 7 Oct 2009 at night Deliver 2 living puppies กำไร

36 Oestrogen and testosterone Research purpose Clinical use is for diagnosis of the presence of gonadal tissue by GnRH stimulating test  Ovarian remnant syndrome oestrogen  Bilateral abdominal cryptorchidism testosterone

37 Ovarian remnant syndrome How to diagnose How to diagnose wait till the bitch/queen shows oestrus signs GnRH stimulation test Adverse effects Adverse effects ▫ Owner ▫ Animal

38 Bilateral abdominal cryptorchidism How to diagnose How to diagnose ▫ Dog: GnRH stimulation test ▫ Cat: penile spine Adverse effects Adverse effects ▫ Testicular tumour (high incidence) Before castrationAfter castration Testicular tumour

39 Ovarian remnant syndrome Exner et al., Theriogenology. Estradiol measurement after GnRH stimulation as a method to diagnose the presence of ovaries in the female domestic cats GnRH analogue buserelin (Receptal, Intervet) 0.4 microgram/kg IM

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ดาวน์โหลด ppt Clinical use of hormone status determination in canine and feline reproduction Suppawiwat Ponglowhapan, DVM (Hons), M.S., M.Sc, PhD Department of Obstetrics.

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